LATEST PUBLICATIONS

Light-Induced Orthogonal Fragmentation of Crosslinked Peptides

JACS Au, 2023
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by Lars Kolbowski, on 17 August 2023, in Chemistry, Crosslinking, Publication

Dual-Bioorthogonal Catalysis by a Palladium Peptide Complex

Journal of Medicinal Chemistry, 2023
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by Ana Perez, on 09 March 2023, in Chemistry, Publication

Improved Peptide Backbone Fragmentation Is the Primary Advantage of MS-Cleavable Crosslinkers

Analytical Chemistry, 2022
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by Lars Kolbowski, on 25 May 2022, in Crosslinking, Publication

Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size-exclusion Chromatography for Proteome-wide Crosslinking Mass Spectrometry

Analytical Chemistry, 2022
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by Ludwig Sinn, on 12 March 2022, in Chromatography, Crosslinking, Ion-mobility spectrometry, Method development, Publication

Leveraging crosslinking mass spectrometry in structural and cell biology

Structure, 2021
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by Andrea Graziadei, on 15 December 2021, in Crosslinking, Crosslinking (in situ), MS development, Publication, Review, Standardisation, Structural Biology (method)

LAB NEWS

Rappsilber laboratory Follow

Our mission is to develop approaches to study protein structure and interactions in cells and make them universally accessible and useful.

RappsilberLab
rappsilberlab Rappsilber laboratory @rappsilberlab ·
6 Jul

#AlphaLink2 goes #ColabFold 😎 @sokrypton #AlphaFold  https://www.biorxiv.org/content/10.1101/2023.06.07.544059v2 https://colab.research.google.com/github/Rappsilber-Laboratory/AlphaLink2/blob/main/notebooks/alphalink2.ipynb

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
12 Jun

In collaboration with the @rbolab, we are pleased to present AlphaLink2 - a modified version of AlphaFold-Multimer that is capable of directly incorporating experimental residue-residue contact data from crosslinking MS (and potentially other techniques as well). (1/7)

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
21 Mar

The AlphaLink-Multimer manuscript is also in progress. We are pleased with the results. Enjoy a sneak peek at what's to come.

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
21 Mar

We and the @rbolab are excited to present AlphaLink https://www.nature.com/articles/s41587-023-01704-z- a modified version of the AlphaFold2 protein structure prediction algorithm that can directly incorporate experimental residue-residue contact data from crosslinking MS (and potentially other techniques)!

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
1 Mar

We discovered a catalytic palladium-binding ribosomal peptide that can simultaneously generate two potent anticancer drugs from inactive precursors and take out lung cancer cells! The future of targeted prodrug therapy gets closer! @adambelsom @ana_aperezl https://pubs.acs.org/doi/10.1021/acs.jmedchem.2c01689

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Funding

We gratefully acknowledge support by our funders especially the Wellcome Trust for their willingness to fund us when our vision was daring but the prospects of ever reaching our goals still far remote. We are also deeply indebted to Edinburgh for providing a very supportive research environment and many great colleagues for being the way they are. This allowed us to ultimately overcome the key challenges of our approach. We also thank the many gifted students of Biotechnology at the TU Berlin for their curiosity and interest in technology development and the TU Berlin for providing a space to tune and scale our approach.