LATEST PUBLICATIONS

Improved Peptide Backbone Fragmentation Is the Primary Advantage of MS-Cleavable Crosslinkers

Analytical Chemistry, 2022
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by Lars Kolbowski, on 25 May 2022, in Crosslinking, Publication

Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size-exclusion Chromatography for Proteome-wide Crosslinking Mass Spectrometry

Analytical Chemistry, 2022
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by Ludwig Sinn, on 12 March 2022, in Chromatography, Crosslinking, Ion-mobility spectrometry, Method development, Publication

Leveraging crosslinking mass spectrometry in structural and cell biology

Structure, 2021
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by Andrea Graziadei, on 15 December 2021, in Crosslinking, Crosslinking (in situ), MS development, Publication, Review, Standardisation, Structural Biology (method)

Proteinanalytik mittels Crosslinking-Massenspektrometrie

GIT Labor-Fachzeitschrift, 2021
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by Ludwig Sinn, on 26 November 2021, in Crosslinking, Publication, Review

Reliable identification of protein-protein interactions by crosslinking mass spectrometry

Nature Communications, 2021
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by Ludwig Sinn, on 11 June 2021, in Crosslinking, Method development, Publication, Software, Structural Biology (application)

LAB NEWS

Rappsilber laboratory Follow

Our mission is to develop approaches to study protein structure and interactions in cells and make them universally accessible and useful.

RappsilberLab
rappsilberlab Rappsilber laboratory @rappsilberlab ·
27 Jul

Happy to share our new preprint, "Protein Complexes in Bacillus subtilis by AI-assisted structural proteomics"! https://www.biorxiv.org/content/10.1101/2022.07.26.501605v1
Fantastic collaboration with the @JorgStulke lab!

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
3 Jun

We present a systematic analysis providing a statistical description of the advantages of MS-cleavable crosslinkers. Using datasets from different labs, we clarify the mechanisms by which these improve crosslinked peptide detection. https://pubs.acs.org/doi/10.1021/acs.analchem.1c05266

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
10 May

Understudied proteins are a big obstacle for biomedical research. Most research to date focuses on a limited set of increasingly well-known proteins, so we are launching the Understudied Proteins Initiative. Find out more @naturemethods (https://rdcu.be/cNbFd) and ...

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rappsilberlab Rappsilber laboratory @rappsilberlab ·
11 Feb

Happy to share our pre-print "SARS-CoV-2 Nsp1 N-terminal and linker regions as a platform for host translational shutoff" - we harness crosslinking-MS to characterize the protein-protein interaction network of SARS-CoV-2 Nsp1 in translational repression.
https://doi.org/10.1101/2022.02.10.479924

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Funding

We gratefully acknowledge support by our funders especially the Wellcome Trust for their willingness to fund us when our vision was daring but the prospects of ever reaching our goals still far remote. We are also deeply indebted to Edinburgh for providing a very supportive research environment and many great colleagues for being the way they are. This allowed us to ultimately overcome the key challenges of our approach. We also thank the many gifted students of Biotechnology at the TU Berlin for their curiosity and interest in technology development and the TU Berlin for providing a space to tune and scale our approach.