MartaTheir structure and interactions define cellular processes and are therefore critical
to our understanding how cells work and how diseases can be cured. Proteins are the active ingredient of life Our mission is to develop approaches to study protein structure
and interactions in cells and make them universally accessible. Learn more LaptopOur mission is to develop approaches to study protein structure
and interactions in cells and make them universally accessible. Learn more Sven&ChristianOur mission is to develop approaches to study protein structure
and interactions in cells and make them universally accessible. Learn more EasysprayOur mission is to develop approaches to study protein structure
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LATEST PUBLICATIONS

Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry

Structure, 2020
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by Andrea Graziadei, on 22 October 2020, in Crosslinking, Crosslinking (in situ), MS development, Publication, Review, Standardisation, Structural Biology (method)

Anatomy of a crosslinker

Current Opinion in Chemical Biology, 2021
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by Adam Belsom, on 25 August 2020, in Chemistry, Crosslinking, Crosslinking (high density), Crosslinking (quantitative), Publication, Quantitation, Review, Standardisation, Structural Biology (method)

In-cell architecture of an actively transcribing-translating expressome

Science, 2020
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by Andrea Graziadei, on 31 July 2020, in Crosslinking, Crosslinking (in situ), Publication, Structural Biology (application), Structural Biology (method)

Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin

Therese Dau, Giulia Bartolomucci, Juri Rappsilber Analytical Chemistry, 2020 https://doi.org/10.1021/acs.analchem.0c00478 Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence coverage when applied to S. pombe whole cell lysates.
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by rapplab-admin, on 06 July 2020, in Method development, Publication

Ultraviolet Photodissociation of Tryptic Peptide Backbones at 213 nm

Lars Kolbowski, Adam Belsom, Juri Rappsilber Journal of the American Society for Mass Spectrometry, 2020 https://doi.org/10.1021/jasms.0c00106 We analyzed the backbone fragmentation behavior of tryptic peptides of a four protein mixture and of E. coli lysate subjected to Ultraviolet Photodissociation (UVPD) at 213 nm on a commercially available UVPD-equipped tribrid mass spectrometer. We obtained 15,178 unique
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by rapplab-admin, on 30 April 2020, in Method development, MS development, Publication

LAB NEWS

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Rappsilber laboratory
RappsilberLabRappsilber laboratory@RappsilberLab·
20 Oct

A great step forward for the field: "Toward Increased Reliability, Transparency, and Accessibility in Crosslinking Mass Spectrometry". Thank you to all colleagues involved! https://doi.org/10.1016/j.str.2020.09.011

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RappsilberLabRappsilber laboratory@RappsilberLab·
21 Aug

You are interested in the "Anatomy of a crosslinker" go-ahead and check out our review in Current Opinion in Chemical Biology 😊https://www.sciencedirect.com/science/article/pii/S1367593120301046 @adambelsom

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FJ_OReillyFrancis O'Reilly@FJ_OReilly·
20 Aug

Our study showed a very different arrangement in Mycoplasma pneumoniae cells.
Taken together these studies show that transcription-translation coupling occurs in distantly related bacteria but remains to be seen how structurally diverse this coupling is.
https://science.sciencemag.org/content/369/6503/554

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FJ_OReillyFrancis O'Reilly@FJ_OReilly·
20 Aug

The two studies out today have a detailed description of how NusG (and also NusA) can bridge the RNA polymerase and ribosome in E.coli. @WebsterMichael0
https://science.sciencemag.org/content/early/2020/08/19/science.abb5036
https://science.sciencemag.org/content/early/2020/08/19/science.abb5317

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FJ_OReillyFrancis O'Reilly@FJ_OReilly·
20 Aug

Exciting new studies out today on transcription translation coupling in Science from the @R_H_Ebright lab and @AlbertW_Lab. @LaurelOld at ASBMB has written about the three papers and talks about all that is yet to be discovered.
https://www.asbmb.org/asbmb-today/science/082020/new-studies-define-transcription-translation-coupl

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Funding

We gratefully acknowledge support by our funders especially the Wellcome Trust for their willingness to fund us when our vision was daring but the prospects of ever reaching our goals still far remote. We are also deeply indebted to Edinburgh for providing a very supportive research environment and many great colleagues for being the way they are. This allowed us to ultimately overcome the key challenges of our approach. We also thank the many gifted students of Biotechnology at the TU Berlin for their curiosity and interest in technology development and the TU Berlin for providing a space to tune and scale our approach.