Rappsilber Laboratory – Innovation in protein mass spectrometry

LATEST PUBLICATIONS

Ultraviolet Photodissociation of Tryptic Peptide Backbones at 213 nm

Lars Kolbowski, Adam Belsom, Juri Rappsilber Journal of the American Society for Mass Spectrometry, 2020 https://doi.org/10.1021/jasms.0c00106 We analyzed the backbone fragmentation behavior of tryptic peptides of a four protein mixture and of E. coli lysate subjected to Ultraviolet Photodissociation (UVPD) at 213 nm on a commercially available UVPD-equipped tribrid mass spectrometer. We obtained 15,178 unique

by rapplab-admin, on 30 April 2020, in Method development, MS development, Publication

The structure of human thyroglobulin

Nature, 2020

by Ludwig Sinn, on 05 February 2020, in Collaboration highlights, Crosslinking, Publication, Structural Biology (application)

Co-regulation map of the human proteome enables identification of protein functions

Nature Biotechnology, 2019

by Georg Kustatscher, on 04 November 2019, in Machine learning, Publication, Software

Lamin A molecular compression and sliding as mechanisms behind nucleoskeleton elasticity

Nature Communications, 2019

by Juri Rappsilber, on 11 July 2019, in Crosslinking, Publication

Quantitative Photo-crosslinking Mass Spectrometry Revealing Protein Structure Response to Environmental Changes

Analytical Chemistry, 2019

by Andrea Graziadei, on 17 June 2019, in Crosslinking, Method development, Publication, Quantitation

LAB NEWS

Rappsilber laboratoryFollow

Our mission is to develop approaches to study protein structure and interactions in cells and make them universally accessible and useful.

Rappsilber laboratory @RappsilberLab·

26 May

[Preprint] We experimentally demonstrate reliable FDR estimation in large scale cross-linking MS studies using the target-decoy approach. Great Work @swantjelenz LudwigSinn @FJ_OReilly https://www.biorxiv.org/content/10.1101/2020.05.25.114256v1 😊

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Rappsilber laboratory @RappsilberLab·

15 May

A thorough analysis of Ultraviolet Photodissociation of tryptic peptide backbones at 213 nm. @LKolbowski @adambelsom 😊https://pubs.acs.org/doi/10.1021/jasms.0c00106

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Rappsilber laboratory @RappsilberLab·

2 Mar

We are happy to see our preprint on the architecture of coupled transcription and translation inside cells online 🥳 Combining the power of whole cell crosslinking-MS, Cryo-ET, and integrative modeling. https://www.biorxiv.org/content/10.1101/2020.02.28.970111v1

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Rappsilber laboratory @RappsilberLab·

10 Feb

What a fruitful cooperation 🙂 First look at the atomic structure of thyroglobulin https://www2.mrc-lmb.cam.ac.uk/first-look-at-the-atomic-structure-of-thyroglobulin/

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Rappsilber laboratory @RappsilberLab·

17 Jan

We are currently looking for feedback on the useability and functionality of the xiVIEW visualization software. If you got some time, please participate 😊 thank you! https://edinburgh.onlinesurveys.ac.uk/xiview-usability

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Funding

We gratefully acknowledge support by our funders especially the Wellcome Trust for their willingness to fund us when our vision was daring but the prospects of ever reaching our goals still far remote. We are also deeply indebted to Edinburgh for providing a very supportive research environment and many great colleagues for being the way they are. This allowed us to ultimately overcome the key challenges of our approach. We also thank the many gifted students of Biotechnology at the TU Berlin for their curiosity and interest in technology development and the TU Berlin for providing a space to tune and scale our approach.